Tuffin et al.demonstrate a method for culturing conditionally immortalized human Door Handle podocytes and GEnCs in 3D by forming spheroid cultures termed GlomSpheres, which are found to segregate in an organized manner.These are Rash Guards shown to establish a GBM-like extracellular matrix with increased cell differentiation and maturity when compared to 2D cultures and their use demonstrated in pharmaceutical screening using rapid imaging approaches.